In infants aged seven days to two months, the observation period has to be extended to 48 hours, during -which two EEGs are taken separated by an interval of 48 hours, both showing electrocerebral silence, i.e., two recordings showing no cerebral activity, one at the beginning of the observation period and another at the end (Table 1).

In infants from two months to one year, an observation period of 24 hours is necessary.  The confirmation should be done by two EEGs separated by 24 hours showing electro-cerebral silence in both, or one EEG with electro­cerebral silence and one cerebral radionucleide angiography (dynamic isotope brain scan) showing no arterial blood flow to the brain.

For children over one year of age, the protocol is not different from that for adults except that the observation period should not be less than 12 hours.

After puberty, protocol for the adult is to be followed.